The FRAP network relies on a set of cutting-edge infrastructures and technology platforms spread across France. These open, shared resources form an essential foundation for supporting fundamental, translational, and clinical research on pancreatic cancer.
The PSEA is a state-of-the-art mice facility offering services for breeding wild-type and genetically modified animals in EOPS-protected areas, free of specific pathogens. Animals are housed in ventilated cages under sterile conditions, with expert staff assisting in genotyping and ES cell injections.
Key features:
Located at the Centre Léon Bérard, the P-PAC platform supports both academic and industrial research in mice and rats, offering essential in vivo imaging tools.
Capabilities:
This facility ensures compliance with French legislation and European Directive 2010/63/EU, hosting cancer research projects, including patient-derived xenografts.
Services include:
A service unit at CRCT, Toulouse focused on functional exploration and phenotypic characterization of animal models.
Highlights:
Dedicated to clinical and preclinical imaging of small animals, FRIM combines advanced imaging modalities with expert support for oncology research.
Capabilities:
Led by Dr. Audrey Vincent, OrgaRES develops 3D tumor organoids from patient samples for rapid chemotherapy and targeted therapy screening.
Highlights:
Supports the development of organoids from multiple cancer types, including pancreatic ductal adenocarcinoma (PDAC).
Equipment includes:
Provides genomic tools for large-scale cancer research, including sequencing, single-cell analysis, and epigenomics.
Key equipment:
Offers high-throughput sequencing and bioinformatics support for both fundamental and translational research.
Features:
Supports genome-wide CRISPR screens, from library preparation to bioinformatics analysis, for functional genomics studies.
Provides custom single-cell technologies including smartseq3, Indrop, 10X Chromium, and chip-seq for transcriptomic analyses.
Specializes in multiplex immunohistochemistry, spatial biology, and cellular imaging with AI-based analysis.
Instruments include:
Equipped with advanced cell sorters and analyzers (BD Influx, Aria Fusion, LSR Fortessa X20, MACSQuant 16).
Services:
Offers full-spectrum cytometry and expert guidance for academic and industrial research.
Specializes in multiplex immunohistochemistry, spatial biology, and cellular imaging with AI-based analysis.
Instruments include:
Performs high-resolution molecular imaging with MALDI mass spectrometry for protein, lipid, and drug mapping at the cellular level.
Provides proteomics, mass spectrometry, and bioinformatics support for biomarker discovery and basic research.
Equipment:
Specializes in multiplex immunohistochemistry, spatial biology, and cellular imaging with AI-based analysis.
Instruments include:
Expertise in production, isolation, and characterization of extracellular vesicles.
Offers BSL-3 oncolytic virus production, custom lentivector and AAV services, and training in viral vector techniques and CRISPR genome editing.
Located within the CRCL facilities, this platform provides multiplex immunofluorescence (IF) imaging services to support preclinical and translational research. The platform enables high-content tissue and cell imaging for cancer and immunology studies.
The CHIC platform offers multiplex IF as part of its spatial biology and tissue imaging services. It supports academic and industrial research, providing instrument access, full-service experiments, and data analysis for complex tissue phenotyping.
This platform is integrated within the local research infrastructure and will provide multiplex IF capabilities to support cancer, immunology, and translational projects. Details on instruments and services are forthcoming.
The Institut Paoli-Calmettes (IPC) multiplex IF platform supports high-resolution imaging of tissue samples. It focuses on cancer research, offering both instrument access and technical support for complex multiplexed analyses.
Embedded within the CRCT facilities, this platform provides multiplex IF imaging for preclinical studies. It enables detailed spatial analysis of tissue samples to support oncology and translational research programs.